Figure 5.

Enzymatic activities in various tissues of transgenic lines. (a) Enzyme activity in protein extracts of six transgenic Arabidopsis lines expressing EG (E1, E2, E3, E4, E5 and E6) and Xyn (X1, X2, X3, X4, X5 and X6). Enzyme activity was determined as the release of reducing sugars using glucose as standard on 1% CMC for endoglucanase and 1% beech-wood xylan for xylanase after incubation for 10 min at 95 °C. (b) MUCase activity in protein extracts of six western blot positive transgenic Arabidopsis lines expressing EG. Activity was expressed as nmol MU mg⁻¹ TSP min⁻¹. Each data point was determined in triplicate and shown as the average ± standard deviation.