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. 2017 Sep 13;7:11472. doi: 10.1038/s41598-017-11815-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological sections stained with Masson’s trichrome showing the distribution of the glomerular area from the control (a), control + calcitriol (b), losartan (c), and losartan + calcitriol (d) groups at 60 days of age. Scale bar = 50 μm. The population of smaller glomeruli is evidenced in the losartan group. (e) The frequency distribution of the glomerular areas in the groups. Non-Gaussian distribution is observed in the losartan group, and a subpopulation of smaller glomeruli in losartan and losartan + calcitriol can be observed. (f) Mean glomerular area of the control and experimental groups. Scale bar = 50 μm. Data are expressed as mean ± SEM. n = 7–12 animals per group. ***P < 0.001 vs. control; ^### P < 0.001 vs. control + calcitriol; and ^§ P < 0.05 vs. losartan.