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. 2017 Sep 13;7:11472. doi: 10.1038/s41598-017-11815-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolocalization of α-SMA in the renal cortex from the losartan (a), losartan + calcitriol (b), and immunolocalization of cubilin from the losartan (c), and losartan + calcitriol (d) groups. Scores for α-SMA in the tubulointerstitial area (e) and cubilin (f) in the renal cortex. Scale bar = 50 μm. Western blot analysis of α-SMA and α1- tubulin (g) in renal cortex from control (lane 1), control + calcitriol (lane 2), losartan (lane 3) and losartan + calcitriol (lane 4) groups. Densitometry of α-SMA (h). A densitometric ratio between the densitometry of α-SMA and α1-tubulin was calculated and data are expressed in comparison to the control, with the mean (±SEM) control value designated as 100%. Blots are representative images from independent experiments (n = 5–6 for each group). Immunohistochemical data are expressed as median and interquartile range (25^th–75^th) and Western blot data are expressed as mean ± SEM (h). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control; ^# P < 0.05 and ^## P < 0.01 vs. control + calcitriol; and ^§ P < 0.05 vs. losartan. Note the increase in α-SMA and decrease in cubulin in losartan groups, which shows lack of cell differentiation.