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. 2017 Sep 13;7:11470. doi: 10.1038/s41598-017-11869-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of 5 or 10 days of ELF-MF exposure (1 mT, 50 Hz) on the protein levels of NRF2-phosphoS40 in SH-SY5Y human neuroblastoma cells. Empty histograms represent unexposed cells (sham, CTR), whereas gray histograms represent ELF-MF-exposed (TRT) cells. Representative grouping of Western blots were reported in panel a, with clear white space delineation between the protein of interest (i.e., NRF2-phosphoS40) and the loading control (i.e., β-actin). T5 and T10, exposed cells for 5 and 10 days, respectively (C5 and C10, time-matched unexposed controls). Representative photographs of immunofluorescence microscopy were reported in panel b (bar = 40 µm). Western blotting-related results were expressed as means ± s.d. Immunofluorescence-related results were expressed as medians with interquartile ranges. *P < 0.05, **P < 0.001 (2 × 2 factorial ANOVA followed by post-hoc Newman–Keuls/Tukey tests for multiple comparison). ***P < 0.001 (Mann-Whitney rank sum tests).