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. 2017 Sep 13;7:11480. doi: 10.1038/s41598-017-11915-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Syk is critical for FcRγ-mediated phagocytosis. (a and c) Phagocytic activity in U937 (Parental) and knockout (KO) cells in which the indicated gene was targeted. After the cells were incubated with opsonised zymosan for 0 min (dotted line) or 60 min (solid line), the fluorescence intensity of the cells was measured by flow cytometry. Histograms from each cell line were overlaid (Overlay) to compare the phagocytic activities. Data are representative of three independent experiments. Similar results were obtained from both replicate cell lines (Supplementary Fig. S2). (b and d) Quantitative analyses of relative phagocytic activity. The percentage of zymosan positive cells (% of phagocytic cells) was compared with data obtained from the Parental cells. The relative values obtained from the indicated cells are expressed as relative phagocytic activity. Results are shown as the mean ± SD of three independent experiments. Similar results were obtained from both replicate cell lines. *P < 0.01 was considered statistically significant. N.S. = not significant.