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. 2017 Sep 13;7:11480. doi: 10.1038/s41598-017-11915-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3BP2 is critical for chemokine mRNA expression induced by cross-linking of FcγRI. U937 (Parental), Syk-knockout (Syk-KO) and 3BP2-knockout (3BP2-KO) cells were stimulated without (−) or with (+) the cross-linking of FcγRI (F(ab′)₂) for 60 min. Total RNA was recovered from these cell lines. (a) Heat map of genes whose expression was increased more than 2-fold in the Parental cells in response to the cross-linking of FcγRI. Expression levels are coloured blue for low intensity and red for high intensity. The gene symbols are indicated on the right. Fold changes observed in each cell line are listed online in Supplementary Table S2. (b) Quantitative analyses of chemokine mRNA expression induced by the stimulation of FcγRI. Real-time PCR was performed to quantitate mRNA expression for IL-8, CCL3L3 and CCL4L2 in the Parental, Syk-KO and 3BP2-KO cells stimulated without (−) or with (+) the cross-linking of FcγRI (F(ab′)₂). The expression relative to that observed in unstimulated cells was shown. Data are representative of three independent experiments and are presented as the mean ± SD. Similar results were obtained using both replicate cell lines (Supplementary Fig. S2). *P < 0.01 was considered statistically significant.