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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: ACS Appl Mater Interfaces. 2017 Feb 16;9(8):6772–6781. doi: 10.1021/acsami.6b14049

Figure 3.

Figure 3

Representative labeling results of MSN(3 nm pores)–SH (600 nmol [–SH]/mL) and MSN(3 nm pores)–NH2 (520 nmol [–NH2]/mL) with *As(OH)3 in 0.1 M HEPES, 70 mM HA, 3 mM EDTA solutions. (a) Labeling temperature was set to be 21 °C. (b) Labeling temperature was set to be 80 °C. (c) Labeling results of MSN(3 nm pores)–SH (600 nmol [–SH]/mL) with *As(OH)3 in 0.1 M HEPES, 70 mM HA, 3 mM EDTA solutions, under varied pH conditions, pH 5.5, 7.5, and 9, at 21 °C. (d) Labeling results of 490 nmol (–SH)/mL MSN(3 nm pores)–SH with *AsO(OH)3 in 0.1 M HEPES, 0.14 M HA, 7 mM EDTA solutions at pH 7.5, 21 °C. While uncertainties were not quantified in these radio-TLC studies, the methods resulted in high uncertainty estimated to be ~15% in solutions containing nearly 0% *As(III) or *As-MSN or 100% *As(V).