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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Toxicol Environ Health A. 2017;80(1):53–68. doi: 10.1080/15287394.2016.1238329

Figure 4. NGF immunoreactivity in TiO2-exposed airway epithelia.

Figure 4

NGF immunoreactivity increased in human nasal, bronchial, and alveolar epithelial cells exposed to 10 μg/ml of TiO2-NP for 24 hr. Immunostaining was done with rabbit polyclonal NGF antibody and Alexa-Fluor 488 conjugated secondary anti-rabbit antibody. Nuclei were stained with DAPI. Cells were visualized using an EC Plan-Neofluar 1.30 oil DIC M27 objective. Magnification = 40X.