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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Toxicol Environ Health A. 2017;80(1):53–68. doi: 10.1080/15287394.2016.1238329

Figure 6. ProNGF/p75NTR signaling.

Figure 6

Top panel shows Western blot and densitometry measurements of dimeric forms of NGF (proNGF, 27kDA) in nasal (A), bronchial (B), and alveolar (C) epithelial cells exposed to 10 μg/ml of TiO2-NP for 24 hr. Middle panel (D) shows the interaction between proNGF and its p75NTR receptor in bronchial epithelial cells exposed to TiO2-NP with the corresponding densitometry analysis. Lower panel (E) shows the Western blot analysis of downstream stress-associated protein kinase/Jun-amino-terminal kinase (SAPK/JNK) in bronchial epithelial cells. Blots were probed with phospho SAPK/JNK [Thr183/Tyr185] and re-probed with JNK antibody as an internal control. Densitometry data are expressed as percentage of non-exposed controls after normalizing with GAPDH for NGF or with total JNK for p-JNK in corresponding graphs. Data shown are the means ± SDs of 3 independent experiments. *p<0.05 compared to non-exposed control cells (C).