FIG 5 .

14-3-3 knockdown alters actin extractability and increases nuclear size. (A) Detergent-extractable actin is increased by 14-3-3 knockdown (KD). E, extracted; P, cell pellet/nonextracted. (B) The plot is normalized to extracted actin from the matching control; results are from three independent experiments. Error bars represent SD. *, P < 0.05. (C) Actin (green) and DAPI (blue) staining from control and 14-3-3 KD. Nuclear size is variable in 14-3-3 KD cells. A magnified view (dashed cyan box) shows that although filaments are smaller in the cytoplasm, the large nuclei have robust filaments that span the width of the nuclei. (The white arrowhead points to the nuclear actin filament, which is also apparent in Fig. 2E.) Scale bar, 2 µm for magnified views. (D) Quantification of nuclear area indicates a 16.4% average increase in 14-3-3 KD cells compared to controls (control, n = 60; KD, n = 96). **, P < 0.01.