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Rac signaling modulates 14-3-3–actin complex formation. (A) Actin filaments (green) are more prominent in tet-induced HA-RacCA-expressing cells. Note that the tet promoter is leaky and some expression is detected in uninduced control cells (images scaled equally). (B) Immunoprecipitation of actin with 14-3-3–VSVG from uninduced (−) and induced (+) HA-RacCA cell lines and quantification of actin binding from three independent experiments. ***, P < 0.001. Scale bar, 5 µm.
