Figure 5.

Maternal IE exposure increases thyroid oxidative stress. Rat dams received distilled water (Control Group; C); distilled water supplemented with 0.6 mg/L (Iodine excess group; 5×) or distilled water supplemented with 7.3 mg/L (Iodine excess group; 50×) throughout pregnancy and lactation. Protein carbonylation measurement was performed through Western blotting analysis, using Ponceau staining as loading control. (A) Left panel: Ponceau S staining of the nitrocellulose membrane. Right panel: Immunoblots obtained after the incubation with primary and secondary specific antibodies to detect carbonylated proteins. (B) The bar graph represents the quantification of blot densitometry analysis normalized to Ponceau S staining. Results are expressed as means ± s.e.m. in percentage (%) (n = 6 per group). *P < 0.05, **P < 0.01 vs C; ^••P < 0.01 vs 5× (ANOVA, Student–Newman–Keuls).