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. 2017 Sep 13;16:152. doi: 10.1186/s12934-017-0768-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — SDS-PAGE gel of cell-free cultivation broth taken at different time points during FB3. Lane 1, protein ladder; lane 2, sample after batch on glycerol; lanes 3–5, samples during fed-batch at q_s,gly = 0.212 g g⁻¹ h⁻¹ (samples were taken every 2 h); lane 6, sample after methanol fed-batch; lane 7, sample after mixed feed phase. Size CBH2 = ca. 60 kDa