Table 1.
Primer | Sequence (5′ - 3′) | Length |
---|---|---|
F3 | GAAATTCTAAGATTTTCTGGAGAC | 24 |
B3 | AGTTTTCCCGTGTTGAGT | 18 |
FIP | TATGGTTAAGATTACGACGGTATCTG-RCAACTGCGAAGGCATW | 43 |
BIP | TGGGTTCTGGGGCGAGTATT-CAAATTAAGCCGCAAGCT | 38 |
LF | CACTCCCTTAACTTTCGTTCTTGAT | 25 |
LB | CGCGCAAGCGAGAAAGTTA | 19 |
F3 and B3, outer primers; FIP and BIP, inner primers; LF and LB, loop primers
The FIP primer consisted of F2 and the complementary strand of F1 (F1c)
The BIP primer consisted of B2 and the complementary strand of B1 (B1c)