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. 2017 Sep 13;14:186. doi: 10.1186/s12974-017-0959-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Double staining for PI with NeuN (neuron marker), Iba-1(microglia marker), GFAP(astrocyte marker), and caspase-1, and ultrastructural changes of necrotic cells. a Double staining for PI with NeuN (neuron marker), Iba-1(microglia marker), and GFAP(astrocyte marker). Scale bar = 50 μm. b Double staining for PI with caspase-1 in the ipsilateral cortex at 24 h after SAH. Scale bar = 50 μm. c Ultrastructural changes of necrotic cells at 24 h after SAH. Neurons in the left panel appeared normal with nuclei, mitochondria, and cytomembranes. Neurons in the right panel showed fragmented nuclei (black asterisk), swollen mitochondria (red asterisk), disrupted cytomembranes (white arrow), damaged organelles (white arrow), and double-membrane autophagosomes (red arrow). Scale bar = 2 μm