Table 4.

Comparative summary of sequencing depth, reads length, and quality between GS FLEX, PGM, and MiSeq platforms

Platform	Raw reads	Filtered readsa	Mean Length after filtering (bp)	Percentage of reads kept after filtering	Mean quality score before quality filtering	Number of identified OTUs
Roche 454	118,018	113,306	377	96%	37.7	1028
Ion Torrent PGM	481,593	71,652	297	14.9%	23.4	2747
Illumina Miseq	4,149,441	3,811,042	334	91.8%	37.5	3731

^aNumber of reads after filtering, reads of less than 200 nucleotides and with quality scores below 25 were removed