Table 2.
Amino acid changes in genes involved in the humoral immune response after CPS immunization
| Gene | PfNF135.C10 | PfNF166.C8 | ||
|---|---|---|---|---|
| Sequence changes | Protein changes | Sequence changes | Protein changes | |
| PF3D7_1036400 LSA-1 |
6 | 4 | 14 | 9 |
| PF3D7_0108300 Conserved unknown | 21 | 12 | 15 | 8 |
| PF3D7_1033100 AdoMetDC/ODC |
10 | 9 | 9 | 8 |
| PF3D7_0108300 MSP2 |
13 | 7 | 8 | 7 |
| PF3D7_0304600 CSP |
6 | 6 A98G S301N K317E E318Q N321K A361E |
7 | 7 S301N K314Q K317E E318K N321K K322T E357Q |
| PF3D7_0509400 RNApol |
6 | 4 | 9 | 6 |
| PF3D7_0630600 Conserved unknown |
7 | 2 | 8 | 3 |
| PF3D7_0502400 MSP8 |
2 | 1 | 4 | 3 |
| PF3D7_1147800 MAEBL |
2 | 0 | 3 | 1 |
| PF3D7_0703700 Conserved unknown |
0 | 0 | 1 | 1 |
| PF3D7_0829000 Conserved unknown |
0 | 0 | 1 | 1 |
| PF3D7_1212700 EIF3A |
1 | 0 | 1 | 0 |
Alterations in gene and protein sequences, based on whole genome sequencing, for 12 genes from NF135.C10 and NF166.C8 were compared to NF54. Single nucleotide polymorphisms and small insertions/deletions are shown together as sequence changes with expected changes in the in silico-translated protein sequence (counting altered amino acids). Changes in CSP protein sequence are indicated with single-letter amino acid code