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. 2017 Sep 13;17:195. doi: 10.1186/s12866-017-1102-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Live cell imaging of infected A549 cells pre-loaded with LysoTracker Red. a M. abscessus ATCC 19977 and (b) M. smegmatis infection in A549 cells for 36 h. Time of image acquisition is depicted as days:hours:minutes:seconds (d:hh:mm:ss) and image acquisition started after 2 h of infection. c Mean fluorescence intensity of LysoTracker Red quantified from square regions in A and B. Total of 5 cells containing phagosomes were analyzed per condition. Bar: 10 μm