Table 1.

Primers and qPCR details for analysis of mRNA levels.

Gene	Sequence (5′→3′)	Product size (bp)	40 PCR cycles
GCLC (BC081702)	F: AAATCAGTAAGTCTCGGTATG	179	95 °C for 15 s
	R: AAGGGTCAGTGGGTCTC		60 °C for 30 s
			72 °C for 30 s
NQO1 (NM_017000)	F: CCATTCCAGCCGACAAC	199	95 °C for 15 s
	R: AGCCGTGGCAGAACTATC		60 °C for 30 s
			72 °C for 30 s
Nrf2 (NM_031789)	F: TTTGGAGGCAAGACATAG	253	95 °C for 15 s
	R: TGGGCAACCTGGGAGTA		60 °C for 30 s
			72 °C for 30 s
HO-1 (NM_012580)	F: TTCACCTTCCCGAGCAT	110	95 °C for 15 s
	R: GCCTCTTCTGTCACCCTGT		60 °C for 30 s
			72 °C for 30 s
β-actin (NM_031144)	F: CGTTGACATCCGTAAAGACC	108	95 °C for 15 s
	R: GGAGCCAGGGCAGTAATCT		60 °C for 30 s
			72 °C for 30 s