Table 1.
Listing of primers used to examine changes in osteoclast gene expression.
| Gene | Role | Forward primer (5′–3′) | Reverse primer (3′–5′) |
|---|---|---|---|
| β2MG (NM_004048) | Reference | TCTCTCTTTCTGGCCTGGAG | CAACTTCAATGTCGGATGGA |
| CtsK (NM_000396.3) | Differentiation | TTCCGCAATCCCGATGGAATA | GCAAAGCTCACCACAGGTAG |
| PICALM (NM_001206946.1) | Endocytosis | ATTTTCTACCCCTAGTTCTTC | AGTAGCAGAGAAAGGATCTC |
| Nqo1 (NM_000903.2) | Oxidative stress | AGCTGGAAGCCGCAGACCTTG | TGAACACTCGCTCAAACCAGCC |
| CHOP (NM_001195053.1) | ER stress | ACCCTGCTTCTCTGGCTTGGC | GGAGAGTGAGGCCTCTGGGAG |
β2MG = beta-2-microglobulin, CtsK = cathepsin K, PICALM = phosphatidylinositol binding clathrin assembly protein, Nqo1 = NADPH quinone oxidoreductase 1, CHOP = C/EBP homologous protein.