Table 3.

Frequencies of mutant spots on wings of D. melanogaster MH descendants derived from standard (ST) and high bioactivation (HB) crosses, after chronic treatment of larvae with different concentrations of EOP.

Genotypes and concentration (%)	Number of flies (N)	Spots per fly (number of spots) statistical diagnosisa
		Small single spots (1⿿2 cells)bm = 2			Large single spots (>2 cells)bm = 5			Twin spots m = 5			Total spots m = 2			Spots with mwh clonesc (n)	Frequency of clone formation per 105 cells per cell divisiond (n/NC)e
mwh/flr3		Fr	N	D	Fr	N	D	Fr	N	D	Fr	N	D			Cf
ST
DXR	40	1.95	(39)	+	1.50	(30)	+	1.35	(27)	+	4.80	(96)	+
Negative control	20	0.70	(14)		0	(0)		0	(0)		0.70	(14)		14	1.43
EOP (0.05)	40	0.18	(7)	⿿	0	(0)	i	0.03	(1)	i	0.20	(8)	⿿	08	0.41	⿿1.02
EOP (0.1)	40	0.18	(7)	⿿	0.08	(3)	i	0	(0)	i	0.25	(10)	⿿	10	0.51	⿿0.92
EOP (0.2)	40	0.38	(15)	⿿	0	(0)	i	0.05	(2)	i	0.43	(17)	⿿	17	0.87	⿿0.56
HB
DXR	40	0.80	(16)	+	0.70	(14)	+	0.60	(12)	+	2.10	(42)	+
Negative control	40	0.60	(24)		0.05	(2)		0	(1)		0.65	(26)		26	1.54
EOP (0.05)	40	0.70	(28)	⿿	0.05	(2)	i	0	(0)	i	0.75	(30)	⿿	30	1.33	0.20
EOP (0.1)	40	0.45	(18)	⿿	0.13	(5)	i	0.03	(1)	i	0.40	(16)	⿿	15	1.43	⿿0.56
EOP (0.2)	40	0.68	(27)	⿿	0.13	(5)	i	0.05	(2)	i	0.85	(34)	i	34	1.54	0.41

^aStatistical diagnosis (D) according to Frei and Würgler [21]: +, positive; w+, weakly positive; ⿿, negative; i, inconclusive. m, multiplication factor for assessment of significantly negative results. Significance levels: α = β = 0.05 when compared with respective control. Fr: frequency. N: number of spots.

^bIncluding rare flr³ single spots.

^cConsidering mwh clones from mwh single and twin spots.

^dOnly single mwh spots were observed in heterozygous mwh/TM3 individuals, since TM3 balancer chromosome does not carry the mutant flr³ gene.

^eInduction frequencies corrected for spontaneous incidence estimated from negative controls.

^fC = 48 800 (approximate number of examined cells per fly).