Fig. 3.

(A) DNA fragmentation pattern on agarose/EtBr gel. Lane 1 marker (1 kb DNA ladder), lanes 2, 3, 4 and 5 DNA isolated from normal, CUR treated, STZ administered, CUR post treated rats, respectively. Arrows indicate ladder formation. CUR decreased all the STZ-induced pro-apoptotic events in hepatic tissue. (B) Measurement of apoptosis in hepatocytes by the use of flow cytometry analysis. Percent distribution of apoptotic and necrotic hepatocytes shown. Cell distribution analysed using Annexin V binding and PI uptake. The FITC and PI fluorescence measured using flow cytometer with FL-1 and FL-2 filters, respectively. Results expressed as dot plot representing as one of the six independent experiments. The measurements were performed six times.