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Normal sample profile for mtDNA and nDNA products. In panel A, PCR conditions were optimized to produce amplicons of different sizes and intensities for 12SmtDNA (177 bp, lane 1) and 18S nDNA (219 bp, lane 2), where the same volume of PCR solutions were loaded in the gel. Panels B and C show the optimized qPCR profiles for the same sample amplification curves and melt-curves with (a) and (b) denoting the qPCR product of nDNA and mtDNA, respectively.
