Table 2.
Measurement of DNA damage-related parameters obtained by alkaline comet assay in HCT 116 and fibroblast cells following exposure to AFT medium. Data are expressed as the mean with SD of three independent experiments. The probability in the ANOVA one-way test for the difference between controls and each treatment is given in parentheses: ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.
| Cell treatment | Concentration (μM) | % DNA in tail | Tail length (pixels) |
|---|---|---|---|
| HCT116 | |||
| Control | 18.09 ± 3.52 | 47.61 ± 3.56 | |
| HQ 24 h | 0 | 17.77 ± 4.50 (ns) | 38.01 ± 3.88 (ns) |
| 33.6 | 16.06 ± 4.30 (ns) | 40.10 ± 3.24 (ns) | |
| 86.3 | 43.61 ± 7.86 (***) | 93.26 ± 5.81 (***) | |
| 108.1 | 57.77 ± 7.43 (***) | 110.00 ± 5.51 (***) | |
| 274.3 | 73.97 ± 7.63 (***) | 153.70 ± 8.64 (***) | |
| Fibroblasts | |||
| Control | 10.22 ± 1.05 | 35.09 ± 0.93 | |
| HQ 24 h | 0 | 5.58 ± 0.84 (***) | 21.89 ± 0.94 (**) |
| 33.6 | 6.47 ± 1.06 (***) | 29.10 ± 1.30 (ns) | |
| 86.3 | 11.03 ± 1.90 (ns) | 27.40 ± 1.11 (ns) | |
| 274.3 | 87.26 ± 5.50 (***) | 506.00 ± 39.28 (***) | |
HQ, hydroquinone.