Fig. 3.
Effects of Cr(VI) on ROS and MMP in HepG2 cells. (A) Representative fluorescent images of HepG2 cells, after staining with cationic fluorescent dye, 2′,7′-dichlorodihydrofluorescein diacetate (H2-DCFDA). (B) Representative fluorescent images of HepG2 cells, after staining with fluorescent dye, JC1. Cells were first grown on glass slide, treated with 12.5 μM and 25 μM Cr(VI) for 24 h followed by incubation with 10 μM H2-DCFDA or 10 μM JC1 at 37 °C for 15 min at 37 °C for 30 min. Then cells were washed with PBS, mounted with fluorescent medium, covered with glass cover slip and observed under a fluorescent microscope. For JC1 staining, the merged images captured under green and red filter were provided. Graphs represent the average green fluorescence intensity ± SEM of separate experiments in each group. “a” indicates the significant difference between the control and Cr(VI) exposed groups where Pa < 0.05.