Fig. 5.

Apoptotic analysis using AO/EB and DAPI staining on H9c2 cells treated pCA, dox and pre/co-administration of pCA after 24 h of treatment. The images were taken using fluorescent microscope (magnification 20×). (A) Control, (B) Dox (1.5 μM), (C) pCA (380 μM), (D) pCA (380 μM) + Dox (1.5 μM) co-administration. (E) Pre pCA (380 μM) + Dox (1.5 μM). The picture is the representation of three separate experiments. H9c2 cells treated with doxorubicin displaying morphological changes such as fragmented nuclei (white solid arrow heads), pyknosis/condensed chromatin (black solid arrow heads) and distorted nuclei of varying nuclear shape and size (white arrows) in comparison to the control/pCA treated cells.