Analysis of ROS formation using DCFH-DA staining on H9c2 cells treated pCA, dox and pre/co-administration of pCA after 6 h of treatment. The images were taken using light (magnification 20×). (A) Control, (B) Dox (1.5 μM) (C) pCA (380 μM), (D) pCA (380 μM) + Dox (1.5 μM) co-administration, (E) pre pCA(380 μM) + Dox (1.5 μM). The picture is the representation of three separate experiments. F shows the mean fluorescent intensity of control and treated groups. *Significant compared to control (p < 0.05), **significant compared to Dox (p < 0.05), #significant compared to pCA and Dox (p < 0.05).