Table 1.
Expression of key CAZy-encoding genes that are induced on the willow substrate
| Gene ID | Annotation a | CAZy family | RPKM | |||
|---|---|---|---|---|---|---|
| ATCC 1015 | CBS513.88 | Glucose 48 h | Willow b 24 h | Willow 24 h + Glucose 5 h | ||
| TID_211595 | An12g04610 | Lytic polysaccharide monooxygenase | AA9 | 0.6 | 246.9 | 1.2 |
| TID_209376 | An07g08950 | eglB - Heat- and alkaline-stable endoglucanase | GH5 | 0.5 | 210.1 | 0.7 |
| TID_211544 | An12g05010 | axeA - Acetyl xylan esterase | CE1 | 0.6 | 161.5 | 0.3 |
| TID_53159 | An07g09330 | cbhA - Putative cellobiohydrolase | GH7 | 1.0 | 155.7 | 1.2 |
| TID_54490 | An12g02220 | Putative cellulose 1,4-beta-cellobiosidase | CBM1, GH6 | 0.3 | 145.2 | 0.4 |
| TID_205580 | An01g11670 | Endoglucanase A | GH5, CBM1 | 0.2 | 120.6 | 1.6 |
| TID_211053 | An14g02760 | eglA - Putative secreted endoglucanase A | GH12 | 0.4 | 116.3 | 1.4 |
| TID_51773 | An01g11660 | cbhB - Putative cellobiohydrolase B | GH7, CBM1 | 0.3 | 115.9 | 10.8 |
| TID_133986 | An08g01760 | Putative cellulase; exocellobiohydrolase | GH6 | 0.2 | 54.9 | 0.5 |
| TID_52011 | An01g03340 | Xyloglucan-specific endo-beta-1,4-glucanase | GH12 | 0.5 | 49.3 | 0.7 |
aThe annotations here are for the A. niger CBS513.88 strain from the AspGD database [44] with the exception that the erroneous endoglucanase annotation for the AA9 family member was corrected.
bAll of the genes listed here have significantly increased expression on the willow substrate compared to the glucose 48 h control (p < 0.05, DESeq).