Fig. 2.

Cytotoxicity and viability screen of PC12 cells exposed to alloy surrogates. (a) Cytotoxicity. PC12 cells treated with and without NGF were exposed to selected concentrations of soluble metal ions and incubated for 18 h in duplicate. The luminescent is reported as relative luminance units (RLU ± SD). Negative control was 200 μM sodium acetate. (b) Viability. PC12 cells treated with or without NGF were exposed to soluble metalions for 18 h in duplicate wells. The fluorescent signal was measured using a plate reader and is reported as mean relative fluorescence units (RFU ± SD). In cells treated with NGF to stimulate differentiation, the cytotoxicity of PC12 cells exposed to the metals was similar to the cells not treated with NGF. Exposure conditions that resulted in a greater than 20% loss in viability are indicated with an open diamond; treatments that resulted in a greater than 60% loss in viability are indicated with closed diamonds.