Fig. 3.

Differences in PC12Cell Gene Expression After 24-h exposure to single metal salts and metal salt combinations. Red horizontal dashed line indicates the significance cutoff for the False Discovery Rate (FDR) analysis. Transcripts that are shared between the treatment scenarios are labeled green (down regulated) and red (up-regulated). (a) A volcano plot comparing the genes expressed in the untreated negative control to the NGF treated positive control. (b)⿿(h) Volcano plots comparing the gene expression differences between the metal treated and positive control (NGF only) treatments. Metals are at the following concentrations: 3 μg W/mL, 2 μg Ni/mL and 1 μg Co/mL. (b) Ni; (c) W; (d) W/Ni; (e) Co; (f) W/Co; (g) Ni/Co; (h) W/Ni/Co. Blue arrow indicates heme oxygenase 1 (HMOX1).