Fig. 2.

Inhibition of N. naja venom caseinolytic activity by L. aspera methanolic extract. 50 μg of N. naja venom was pre-incubated with various concentrations of plant extract (w/w) for 1 h at 37 °C. The protease activity was assayed in SDS-PAGE method. Lane 1: 50 μg of venom; Lane 2: casein; Lane 3: casein incubated with venom; Lane 4 to 12: samples containing casein incubated with venom in presence of different concentrations of plant extract i.e. 1:05, 1:10, 1:20 to 1:80 (w/w venom: plant extract) respectively. Arrows indicate the major bands of casein. Lane 1–3 served as controls.