Fig. 5.

Inhibition of N. naja venom hyaluronidase activity by L. aspera methanolic extract. 100 μg of N. naja venom was pre-incubated with various concentrations of plant extract (w/w) for 1 h at 37 °C. The hyaluronidase activity was assayed in substrate gel SDS-PAGE zymogram under non-reducing conditions. Lane 1 and 8: 100 μg of N. naja venom in absence of plant extract, indicating the presence of two isoforms of hyaluronidase; Lane 2 to 7: 100 μg of venom in presence of different concentrations of plant extract i.e. 1:05, 1:10, 1:25, 1:50, 1:75 and 1:100 (w/w venom: plant extract), respectively, showing dose dependent inhibition of hyaluronidase activity of both the isoforms. Arrows indicate two isoforms of venom hyaluronidase.