Table 3.
Genes differentially regulated by exposure to 20nmAgCit or Ag+.
| Gene |
Category | Fold Δ 20nmAgNP |
Fold Δ Ag+ |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Name | Sequence | #1 4 h |
#2 4 h |
#1 8 h |
#1 24 h |
#2 24 h |
#2 4 h |
#2 24 h |
|
| Early or decreasing response to 20nmAgCit | |||||||||
| clec-7 | F10G2.3 | – | 0.5 | 0.4 | 0.6 | 0.6 | |||
| comt-3 | Y40B10A.2 | Imm [2], Metal [33], [35], Xen/Str [5], [14] | 2.2 | 1.9 | 4.8 | 1.6 | |||
| comt-4 | 40B10A.6 | Imm [29], Metal [60], Xen/Str [5] | 6.9 | 5.5 | 2.4 | 1.8 | 1.6 | 23 | 2.9 |
| cpr-3 | T10H4.12 | Imm [2], [29], [65], [84], Endo/Lyso [3] | 2.2 | 1.6 | 4.3 | 1.8 | |||
| fipr-22 | C37A5.2 | Imm [28] | 14 | 2.4 | |||||
| fipr-23 | C37A5.4 | Imm [28] | 11 | 2.1 | |||||
| folt-2 | F37B4.7 | Imm [29], Metal [35], DR [27] | 0.2 | ||||||
| ftn-1 | C54F6.14 | ↑ Imm [28], Metal [33], Xen/Str [9], [24], DR [27] | 1.7 | 2.4 | 13 | 0.5 | |||
| gst-12 | F37B1.2 | Metal [33], [35], Xen/Str [14], [46], [76] | 3.9 | 2.7 | 2.2 | 1.6 | 2.1 | ||
| gst-30 | ZK546.11 | Metal [33], [35], Xen/Str [14], [76] | 2.4 | 2.3 | 2.0 | 4.3 | |||
| gst-31 | Y53F4B.35 | Metal [33], [35] | 2.7 | 2.5 | 1.6 | ||||
| gst-9 | R05F9.5 | Metal [33], [35], Xen/Str [14], [76] | 3.7 | 2.4 | 1.9 | ||||
| hmt-1 | W09D6.6 | Metal [9], Mitoc [9] | 2.3 | 2.3 | 1.7 | 1.5 | 5.8 | 1.8 | |
| hsp-16.1 | T27E4.8 | ↑ Metal [35], [45], Xen/Str [14], [22] | 0.6 | 5.8 | |||||
| ins-7 | ZK1251.2 | Imm [84], [86] | 1.7 | 5.1 | |||||
| lact-1 | F46H5.8 | Imm [42] | 2.7 | 1.7 | 5.0 | ||||
| msd-1 | F44D12.3 | – | 0.5 | ||||||
| nlp-26 | Y43F8C.2 | – | 6.9 | 4.0 | 3.4 | 1.6 | 5.9 | ||
| numr-1 | F08F8.5 | Metal [33], [35], [60], Xen/Str [14] | 17 | 27 | 5.1 | 1.8 | 1.7 | 81 | 2.4 |
| oac-6 | C31A11.5 | Imm [29], [65], Metal [35], [60], Xen/Str [14] | 1.8 | 6.5 | |||||
| pho-13 | F07H5.9 | Imm [29] | 0.4 | 0.2 | |||||
| pmp-5 | T10H9.5 | Imm [29], Metal [60], Perox [9] | 0.6 | 0.2 | |||||
| sams-1 | C49F5.1 | Imm [29], DR [9] | 0.2 | ||||||
| scl-2 | F49E11.10 | ↑Imm [65], ↓ Imm [42] | 5.3 | 0.4 | |||||
| tsp-1 | C02F5.8 | Imm [29], [65], [84], [85] | 2.0 | 5.8 | 1.6 | ||||
| tsp-10 | T14B4.4 | – | 0.2 | ||||||
| tsp-2 | C02F5.11 | Imm [29], [65] | 1.9 | 5.2 | 1.8 | ||||
| ttr-23 | T21C9.8 | Imm [29] | 1.9 | 1.7 | 9.8 | 2.2 | |||
| ugt-18 | ZC443.5 | Imm [29], [65] | 2.3 | 8.3 | |||||
| ugt-3 | ZC455.3 | Metal [35] | 6.7 | 4.1 | 84 | ||||
| ugt-63 | C04F5.7 | – | 0.1 | ||||||
| ugt-9 | T19H12.1 | Metal [35], Xen/Str [71], [87] | 2.0 | 1.9 | |||||
| B0024.4 | Imm[29], [40], [65], Metal [60], Xen/Str [87] | 2.6 | 2.4 | 2.4 | 11 | ||||
| C05D9.9 | Imm [29] | 5.0 | |||||||
| C17H12.6 | Imm [2], [29], Metal [33], [35] | 1.6 | 2.4 | 1.6 | 2.0 | ||||
| C28C12.4 | Metal [35] | 4.5 | 4.3 | 3.0 | 2.5 | 1.9 | 19 | 5.0 | |
| C42D4.3 | Xen/Str [20] | 8.6 | |||||||
| C45B2.2 | Xen/Str [14] | 3.6 | 2.9 | 2.0 | 5.0 | 1.8 | |||
| C45B2.3 | Metal [35] | 2.1 | 5.2 | 1.6 | 41 | ||||
| C50F7.5 | Imm [29] | 20 | |||||||
| D1086.3 | Imm [29], Metal [35] | 0.6 | 0.4 | 0.6 | 0.3 | ||||
| F01D4.8 | Xen/Str [20] | 7.4 | |||||||
| F23F12.12 | – | 0.5 | |||||||
| F46F2.3 | Imm [29] | 0.2 | |||||||
| F47B8.2 | Imm [65], Metal [35] | 2.5 | 2.4 | 1.8 | 1.6 | 5.0 | 1.7 | ||
| F49H6.13 | Imm [29] | 2.2 | |||||||
| F53A9.2 | Imm [29], Xen/Str [14] | 2.3 | 2.9 | ||||||
| F57H12.6 | Xen/Str [22] | 27 | 21 | 17 | 5.3 | 3.3 | 57 | 7.3 | |
| K01C8.1 | Metal [35] | 0.5 | |||||||
| R07E5.4 | Imm [29], Endo/Lyso [9] | 0.5 | 0.4 | ||||||
| T05E12.6 | Imm [29] | 0.2 | |||||||
| T19D12.4 | Imm [2], [29], Metal [35] | 2.2 | 2.0 | ||||||
| T24C4.4 | Imm [29] | 3.2 | 1.6 | 4.2 | |||||
| T28H10.3 | Imm [47], [65], Endo/Lyso [3] | 6.1 | |||||||
| W03G1.5 | Metal [35] | 2.0 | 1.8 | 10 | |||||
| ZK105.5 | – | 0.5 | 0.6 | ||||||
| ZK742.3 | Metal [60], Xen/Str [14] | 1.8 | 5.6 | ||||||
| ZK742.4 | Metal [33], [35], Xen/Str [14], [76], Lipid [9] | 2.8 | 2.3 | 1.9 | 1.6 | 1.5 | 2.8 | 1.8 | |
| Late or increasing response to 20nmAgCit | |||||||||
| acdh-1 | C55B7.4 | Imm [2], [29], [47], [84], Metal [35], Lipid [48] predicted to be a mitochondrial enzyme that catalyzes the first step of fatty acid beta-oxidation and thus plays a key role in energy production | 0.5 | 0.2 | |||||
| clec-17 | E03H4.10 | Imm [29], [40], [65], Xen/Str [30] | 1.7 | 2.0 | 1.6 | 2.4 | 1.8 | ||
| clec-60 | ZK666.6 | Imm [36], [83], Metal [35], Xen/Str [87] | 0.5 | 0.3 | |||||
| cnc-2 | R09B5.3 | Imm [29], [39], [41] | 3.4 | 1.8 | 3.5 | 5.0 | 4.0 | 4.8 | 4.1 |
| cnc-4 | R09B5.9 | Imm [28], [29], [41] | 2.3 | 1.7 | 4.9 | 4.1 | 3.7 | 7.7 | 4.4 |
| cnc-6 | Y46E12A.1 | Imm [29], [41], Metal [35] | 1.5 | 2.3 | 9.8 | 6.5 | 18 | 16 | |
| cnc-7 | F53H2.2 | Imm [28] | 1.8 | 1.8 | 3.1 | 6.1 | 3.9 | 11 | 2.9 |
| dhs-25 | F09E10.3 | Imm [29], [65], Mitoc [9] | 0.6 | 0.3 | |||||
| dod-21 | C32H11.9 | Metal [18], [35] | 1.6 | 2.3 | 1.9 | 2.9 | 7.3 | 15 | |
| far-4 | F15B9.2 | Metal [35], Lipid [9] | 1.5 | 3.5 | 7.1 | 6.6 | 8.8 | ||
| fip-1 | F22B7.4 | Imm [41] | 3.5 | 3.4 | 13 | ||||
| fip-5 | F41E7.4 | Imm [9] | 2.0 | 1.6 | 1.9 | 2.1 | |||
| fipr-26 | F53B6.8 | Imm [42], Xen/Str [14] | 1.6 | 1.7 | 11 | ||||
| fmo-3 | Y39A1A.19 | Xen/Str [9], [24] | 1.9 | 2.5 | 2.0 | 2.4 | |||
| lys-4 | F58B3.1 | Metal [60] | 0.4 | 0.3 | |||||
| nlp-25 | Y43F8C.1 | Metal [35] | 2.4 | 2.2 | |||||
| nlp-27 | B0213.2 | Imm [83] | 2.1 | 2.6 | |||||
| nlp-29 | B0213.4 | Imm [39], [47], [84] | 3.1 | 3.1 | 2.9 | 7.0 | 4.0 | ||
| nlp-30 | B0213.5 | Imm [41] | 2.1 | 1.9 | 2.2 | 3.0 | |||
| nlp-31 | B0213.6 | Imm [39], [41] | 2.1 | 2.4 | 2.7 | 1.8 | 3.6 | ||
| oac-46 | T14D7.2 | – | 1.6 | 2.0 | 1.7 | 2.0 | |||
| phi-59 | T19B10.2 | Imm [41], Xen/Str [20] | 2.2 | 3.0 | 2.6 | 2.2 | 3.0 | ||
| pho-6 | F52E1.8 | Metal [35], Endo/Lyso [3] | 2.1 | 2.3 | 4.6 | ||||
| ptr-8 | F44F4.4 | – | 2.0 | 2.5 | 1.9 | 2.2 | |||
| scl-24 | F08E10.7 | Xen/Str [78] | 0.5 | 0.6 | |||||
| spe-11 | F48C1.7 | Metal [35], Xen/Str [76] | 2.3 | 2.6 | 4.8 | ||||
| sqrd-1 | F02H6.5 | Mitoc [9] | 0.5 | 0.6 | 0.6 | ||||
| thn-1 | F28D1.3 | Imm [2], [29] | 0.5 | 0.5 | |||||
| ttr-29 | R90.4 | – | 1.8 | 5.3 | 2.7 | ||||
| ttr-8 | R13A5.6 | – | 2.2 | 2.4 | 1.6 | 2.9 | |||
| C14A6.16 | – | 1.8 | 1.5 | 2.3 | 4.8 | 2.9 | 5.1 | 3.7 | |
| C30F12.3 | – | 1.6 | 2.2 | 3.1 | |||||
| D1086.9 | – | 3.7 | 8.9 | ||||||
| F07B7.8 | – | 6.1 | 4.1 | 12 | |||||
| F13D12.3 | – | 2.1 | 1.5 | 1.6 | 1.9 | ||||
| F16B4.7 | – | 2.4 | 1.7 | 2.5 | |||||
| F21H7.3 | – | 2.1 | 1.6 | 1.8 | |||||
| F26G1.2 | Xen/Str [71] | 2.0 | 1.9 | 2.1 | |||||
| F41C3.2 | – | 1.6 | 2.0 | ||||||
| F45D11.14 | – | 0.4 | 0.6 | 0.4 | 0.4 | ||||
| F52F10.2 | Endo/Lyso [9], [24] | 2.2 | 1.6 | 2.0 | |||||
| F56D3.1 | Xen/Str [22] | 2.2 | 1.5 | 2.4 | |||||
| H39E23.3 | – | 5.1 | 5.8 | 9.4 | |||||
| K11G9.5 | Xen/Str [14], IonT(−) [9] | 2.1 | 1.9 | 1.9 | 1.6 | ||||
| M162.5 | Metal [35] | 1.7 | 3.1 | 2.2 | 2.6 | 2.7 | |||
| T28D6.3 | Metal [35], Xen/Str [30] | 0.4 | 0.2 | 0.6 | |||||
| Y43C5A.3 | Imm [29], Metal [35] | 1.6 | 1.5 | 1.8 | 3.5 | 3.3 | 2.7 | 4.5 | |
| ZK970.7 | Imm [29], Metal [35] | 1.9 | 1.5 | 5.9 | 10 | 7.0 | 29 | 11 | |
| Sustained response to 20nmAgCit | |||||||||
| amt-1 | C05E11.4 | Imm [29], IonT(+) [9] | 0.6 | 0.6 | 0.5 | 0.6 | |||
| clec-74 | Y46C8AL.8 | Imm [29], Xen/Str [76] | 1.7 | 1.9 | 2.0 | 2.0 | |||
| clec-9 | Y70C5C.2 | Metal [33], [35] | 3.1 | 2.4 | 3.0 | 2.0 | 1.7 | 1.8 | |
| cyp-32A1 | C26F1.2 | Imm [29] | 1.7 | 1.8 | 1.8 | 2.0 | 1.9 | 4.5 | 2.2 |
| ech-9 | F01G10.3 | Imm [29], [65], Lipid [48], Perox [9] | 1.8 | 2.4 | 1.8 | 1.5 | 6.6 | ||
| hmit-1.1 | Y51A2D.4 | IonT(+) [9] | 2.2 | 2.4 | 2.3 | 2.0 | 0.5 | ||
| ora-1 | F57H12.3 | – | 29 | 12 | 16 | 20 | 8.8 | 58 | 18 |
| vglu-3 | T07A5.3 | Xen/Str [20] | 1.7 | 1.6 | 2.0 | 1.6 | 1.9 | ||
| C15A11.7 | Imm [29], [65] | 1.6 | 2.0 | 2.5 | 2.5 | 1.9 | 1.9 | ||
| C34F11.8 | Imm [29], [41], [65] | 1.7 | 1.8 | 1.8 | 1.9 | 5.4 | 2.1 | ||
| C49C3.9 | Imm [2], [29] | 1.5 | 2.5 | 2.0 | 1.5 | 1.7 | 4.5 | 2.0 | |
| F59E11.7 | – | 2.2 | 1.6 | 2.1 | 2.4 | 2.2 | 9.2 | 3.2 | |
| R08E5.3 | Imm [65], Metal [35] | 0.5 | 0.4 | 0.7 | 0.6 | 0.6 | |||
| T03F7.7 | – | 1.6 | 2.0 | 1.6 | 4.1 | 2.2 | |||
| T13F3.6 | Imm [29], Metal [35], Xen/Str [30] | 0.6 | 0.6 | 0.6 | 0.5 | 0.7 | 0.3 | ||
| ZK673.1 | Imm [29] | 0.5 | 0.6 | 0.6 | 0.4 | 0.5 | |||
Microarray data indicating differential gene regulation in response to 100 μg/mL 20nmAgCit or 12.5 μg/mL Ag+ for indicated times was compared to published expression/function information in order to assign genes into general response categories. Categories: immune response (Imm), metal response (Metal), xenobiotic or heat stress (Xen/Str), lipid metabolism (Lipid), endosome or lysosome associated (Endo/Lyso), mitochondrial (Mitoc), peroxisomal (Perox), dietary restriction (DR), cation transport (Ion(+)), anion transport (Ion(−)). For genes regulated up for some exposures and down for others, ↑ or ↓indicates category for up or down regulation only. Genes listed in table are limited to those that had fold changes in response to 20nmAgCit ≥ 2 fold, and/or to Ag+ ≥ 5 fold in at least one experiment at any assessed exposure time.