Figure 5.
CLI-095 or pyrrolidine dithiocarbamate down-regulated TLR4 and NF-κB expression in RAW264.7 cells. Twenty-four hours after ferrous myoglobin (200 μmol/L) administration, TLR4 and NF-κB expression in RAW264.7 cells were quantified by Western blotting (a) and reverse transcriptase-quantitative polymerase chain reaction (d). Quantification of TLR4 (b) and NF-κB (c) expression in RAW264.7 cells. *P < 0.01 versus control; †P < 0.01 versus glycerol treated. TLR4: Toll-like receptor 4; NF-κB: Nuclear factor-kappa B.