Fig. 2.
Effect of Iban on BKCa-channel activity in MDCK cells. These experiments were conducted as cells were bathed in high-K+ (145 mM) solution containing 1.8 mM CaCl2. Cell-attached current recordings were made and each cell was held at +50 mV. Followed by standardization with Iban (30 μM), Iban along with NaHS (Iban + NaHS) and PF573228 (Iban + PF573228) were also monitored for the alteration on BKCa-channel activity which was measured at the level of +50 mV. (A) Original current traces obtained with or without addition of Iban at +50 mV. control (left, black); 10 μM Iban (middle, blue) and 30 μM Iban (right, red). (B) Summary of the data showing effects of Iban, Iban plus NaHS and Iban plus PF573228 on the channel open probability. Values are means ± SEM for n = 16–18 cells in each group. *Significantly different from control (P < 0.05) and #significantly (P < 0.05) different from Iban (30 μM) alone. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)