Fig. 6.

Inhibitory effects of Iban and oxalate on the activity of BK_Ca channels recorded from MDCK cells. The experiments were conducted in symmetrical K⁺ solution (145 mM) containing 1.8 mM CaCl₂, and the channels were recorded from cell-attached patched of MDCK cells. The potential was held at +50 mV. (A) Original current traces obtained in control (black) or during the exposure to 10 μM Iban (blue), 30 μM oxalate (red), or 10 μM Iban plus 30 μM oxalate (green). (B) Bar graph showing the effect of Iban (10 μM) and oxalate (30 μM) on the probability of BK_Ca-channel openings. Values are means ± SEM for n = 11–13 cells in each group. In the experiments with Iban plus oxalate, oxalate was subsequently applied after addition of Iban to the bath. ^*Significantly different from control (P < 0.05). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)