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. 2015 Aug 3;2:1075–1085. doi: 10.1016/j.toxrep.2015.07.021

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Esterified cholesterol levels (A) of adrenal gland from vehicle and 10 mg/kg bw/d Roundup^®-treated animals. Adrenal glands were isolated post treatment and lysate was prepared. Esterified cholesterol levels were obtained by subtracting free cholesterol levels from total cholesterol values. qPCR was performed to detect Hsl gene expression in the adrenal gland of vehicle and 10 mg/kg bw/d Roundup^®-treated animals (B). Rpl19 was used as internal control. qPCR value for vehicle was set as 1 fold and Roundup^®-treated group values were plotted in relation to the vehicle treated group. Values are presented in mean ± SEM (n = 6 rats per group). ‘t’ test was performed to compare the two groups.