Fig. 5.

Photomicrography of representative morphological changes of HepG2 cells using May-Grünwald–Giemsa staining. The cells (1 × 10⁶ cells/well) were pretreated with or without the pulp (GPE) or peel/seed extracts (GPSE) of gabiroba fruits (both at 1000 μg/mL) for 1 h followed by exposure to CCl₄ (4 mM) for 6 h. Posteriorly, the medium was exchanged and after 24 h, the cells were stained and morphological changes were analyzed. The images were taken using a 100× objective. Arrows in CCl₄ group indicate DNA fragmentation.