Table 1.
Sequences of primers used for real-time RT-PCR analysis (5′–3′).
| Encoded protein | Forward | Reverse |
|---|---|---|
| Cyclophilin | ACGCCACTGTCGCTTTTC | CTGCAAACAGCTCGAAGGA |
| Gck | CTGGATGACAGAGCCAGGAT | CTCTGCCAGGATCTGCTCTAC |
| Gapdh | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTCATGAG |
| Pklr1 | ACTTAGCAAAGTCTGCTTTAAGTGG | TGGCACGTCTCAGGTATCC |
| Pklr2 | GTGGAGGCTTCCTTCAAGTG | AGGTCGGTAGCGAGACAGAA |
| Acly | GCCCTGGAAGTGGAGAAGAT | CCGTCCACATTCAGGATAAGA |
| ACC1 | GCGTCGGGTAGATCCAGTT | CTCAGTGGGGCTTAGCTCTG |
| ACC2 | TGAATCTCACGCGCCTACTA | GCCTCTCTTCACCAGATGGA |
| Fasn | GCTGCTGTTGGAAGTCAGC | AGTGTTCGTTCCTCGGAGTG |
| Elovl6 | CAGCAAAGCACCCGAACTA | AGGAGCACAGTGATGTGGTG |
| Scd1 | TTCCCTCCTGCAAGCTCTAC | CAGAGCGCTGGTCATGTAGT |
| Gpam | GGAAGGTGCTGCTATTCCTG | TGGGATACTGGGGTTGAAAA |
| Dgat1 | TCGTGGTATCCTGAATTGGTG | AGGTTCTCTAAAAATAACCTTGCATT |
| Dgat2 | GGCGCTACTTCCGAGACTAC | TGGTCAGCAGGTTGTGTGTC |
| ChREBP | GGCCTGGCTGGAACAGTA | CGAAGGGAATTCAGGACAGT |
| Srebf1 | GGTTTTGAACGACATCGAAGA | CGGGAAGTCACTGTCTTGGT |
| Nr1h2 | GCTCTGCCTACATCGTGGTC | CTCATGGCCCAGCATCTT |
| Nr1h3 | TGTGCGCTCAGCTCTTGT | TGGAGCCCTGGACATTACC |
| Ppara | CTGAGACCCTCGGGGAAC | AAACGTCAGTTCACAGGGAAG |
| Pparg | GAAAGACAACGGACAAATCACC | GGGGGTGATATGTTTGAACTTG |
| Ppard | ATGGGGGACCAGAACACAC | GGAGGAATTCTGGGAGAGGT |
| Cidea | AAACCATGACCGAAGTAGCC | AGGCCAGTTGTGATGACTAAGAC |
| Cideb | CTGCCAGCCTCCAAGAACT | TAGCACTCCACGTAGCAGCA |
| Cidec | GATGGACTACGCCATGAAGTC | GTGCTCACTGCCACATGC |
Protein product abbreviations: Gck, glucokinase; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Pklr1 and Pklr2, pyruvate kinase liver and red blood cell (Pklr) 1 and 2; Acly, ATP-citrate synthase; ACC1 and ACC2, acetyl-coenzyme A carboxylase 1 and 2; Fasn, fatty acid synthase; Elovl6, long-chain elongase; Scd1, acyl-CoA desaturase 1; Gpam, glycerol-3-phosphate acyltransferase; Dgat1 and Dgat2, diacylglycerol acyltransferase 1 and 2; ChREBP, carbohydrate-responsive element-binding protein; Srebp1, sterol regulatory element binding factor 1; Nr1h2 and Nr1h3, liver X receptor (LXR) beta and alpha; Ppara, Pparg and Ppard, peroxisome proliferator-activated receptors alpha, gamma, and delta; Cidea, Cideb and Cidec/Fsp27, cell death–inducing DNA fragmentation factor 45-like effectors (CIDEs) A, B, and C.