Table 2. Fermentation end products of R. ilealis CRIBT produced during growth on different carbohydrates (glucose, FOS or L-fucose) or in basal medium in the absence of a carbon source (control condition).
Samples were obtained during mid-exponential phase (∼8–10 h incubation; used for transcriptome analysis) and in stationary phase (24 h incubation). For the control cultures, fermentation products are shown for the individual cultures separating the carbohydrates used for preconditioning of the inoculum. For the three other conditions, values represent means of triplicate cultures with standard deviations.
Formate (mM) | Acetate (mM) | Propionate (mM) | Lactate (mM) | 1,2-propane-diol (mM) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
8–10 h | 24 h | 8–10 h | 24 h | 8–10 h | 24 h | 8–10 h | 24 h | 8–10 h | 24 h | |
Control: basal medium | ||||||||||
(glucose inoc.) | 3.2 | 7.7 | 2.0 | 6.2 | 2.0 | 2.2 | N.D. | N.D. | N.D. | N.D. |
(FOS inoc.) | 4.5 | 9.2 | 2.4 | 7.4 | 2.4 | 2.9 | N.D. | N.D. | N.D. | N.D. |
(L-fucose inoc) | 4.8 | 10.8 | 2.3 | 9.8 | 2.3 | 3.0 | N.D. | N.D. | 1.0 | 1.0 |
Basal medium + glucose (5% w/v) | 4.4 ± 1.2 | 28.2 ± 4.3 | 1.0 ± 0.9 | 16.3 ± 2.2 | 1.0 ± 0.9 | 1.3 ± 0.1 | N.D. | 3.0 ± 0.7 | N.D. | N.D. |
Basal medium + FOS (5% w/v) | 4.7 ± 0.6 | 27.3 ± 2.5 | 1.4 ± 0.0 | 17.7 ± 1.4 | 1.4 ± 0.0 | 1.6 ± 0.1 | N.D | 2.5 ± 0.3 | N.D. | N.D. |
Basal medium + L-fucose (5% w/v) | 6.7 ± 0.1 | 19.5 ± 3.6 | 2.8 ± 0.1 | 16.3 ± 2.9 | 2.8 ± 0.1 | 2.8 ± 0.4 | N.D. | N.D. | 1.3 ± 0.1 | 7.7 ± 1.4 |
Notes.
- N.D.
- not detected