Table 5.
Changes in the activities of enzymatic antioxidants in control and experimental rats.
| Groups | Control | Normal + HDN (80 mg/kg) | Normal + Fe (30 mg/kg) | Fe (30 mg/kg) + HDN (80 mg/kg) |
|---|---|---|---|---|
| SOD | ||||
| Liver | 7.74 ± 0.56a | 8.30 ± 0.81a | 5.45 ± 0.32b | 6.75 ± 0.45c |
| Kidney | 12.07 ± 0.92a | 12.51 ± 1.06a | 8.09 ± 0.56b | 10.41 ± 0.73c |
| CAT | ||||
| Liver | 90.51 ± 6.67a | 94.42 ± 5.92a | 55.04 ± 4.13b | 75.21 ± 5.00c |
| Kidney | 48.25 ± 4.06a | 51.07 ± 4.28a | 32.11 ± 1.91b | 40.34 ± 3.19c |
| GPx | ||||
| Liver | 7.03 ± 0.46a | 7.28 ± 0.49a | 4.61 ± 0.25b | 6.02 ± 0.32c |
| Kidney | 5.46 ± 0.35a | 5.52 ± 0.48a | 3.22 ± 0.16b | 4.76 ± 0.29c |
| GST | ||||
| Liver | 7.98 ± 0.46a | 8.19 ± 0.40a | 5.95 ± 0.26b | 7.08 ± 0.34c |
| Kidney | 6.09 ± 0.34a | 6.15 ± 0.42a | 3.97 ± 0.28b | 5.54 ± 0.28c |
Values are mean ± SD for 6 rats in each group. Values are not sharing a common superscript letter (a, b and c) differ significantly at p < 0.05 (DMRT). SOD–one unit of enzyme activity was taken as the enzyme reaction, which gave 50% inhibition of NBT reduction in one minute/mg protein; CAT—μmol of H2O2 consumed/min/mg protein; GPx—μg of GSH consumed/min/mg protein; GST—μmoles of CDNB-GSH conjugate formed/min/mg protein. HDN—hesperidin, Fe—ferrous sulfate.