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. Author manuscript; available in PMC: 2018 Jul 11.
Published in final edited form as: Biochemistry. 2017 Jun 30;56(27):3507–3517. doi: 10.1021/acs.biochem.7b00268

Figure 4.

Figure 4

In the context of a peptide, S-GlcNAc is completely stable against human OGA enzymatic deglycosylation. O-GlcNAcylated (blue) or S-GlcNAcylated (orange) peptides (50 μM, in PBS at pH 7.4) were incubated with human OGA (1 μM) at 37 °C for ≤72 h. The S-GlcNAcylated peptide HPLC trace is offset in the y-direction for the sake of clarity. mAU indicates milliabsorbance units. RP-HPLC conditions were 0–70% buffer B over 60 min; buffer A consisted of 0.1% TFA in H2O, and buffer B consisted of 0.1% TFA and 90% ACN in H2O.