Fig. 3.

Intracellular ROS generation in HeLa cells exposed to Ni NWs. (A) Fluorescence microscope (200×) of cells treated with various concentrations of Ni NWs for 24 h. Cells were incubated with DCFH-DA and washed before observation. (B) Fluorescence intensity detected using flow cytometry for HeLa cells treated with Ni NWs at the concentration of 10,000 NWs per cell for 24 h. Cells treated with 0.8 mmol/L H₂O₂ served as positive control.