Table 2.
Effect of co-exposure of arsenic and nicotine on brain oxidative stress variables in rats.
| Normal | Arsenic | Nicotine | As + Nicotine | |
|---|---|---|---|---|
| ROS | 272.7 ± 1.7 | 285.4 ± 0.7* | 293.6 ± 5.9* | 308.3 ± 0.4*, ** |
| GSH | 5.48 ± 0.08 | 4.65 ± 0.11* | 5.31 ± 0.14 | 6.16 ± 0.20*, ** |
| GSSG | 2.13 ± 0.03 | 1.92 ± 0.14 | 2.64 ± 0.09 | 2.81 ± 0.20*, ** |
| GSH/GSSG | 2.59 ± 0.03 | 2.20 ± 0.08* | 2.09 ± 0.09* | 2.07 ± 0.11* |
| TBARS | 2.43 ± 0.09 | 2.83 ± 0.24 | 2.87 ± 0.18 | 3.04 ± 0.13 |
| SOD | 2.09 ± 0.23 | 1.42 ± 0.09* | 1.28 ± 0.14* | 0.876 ± 0.08* |
| Catalase | 4.19 ± 0.14 | 3.16 ± 0.34* | 3.00 ± 0.10* | 3.05 ± 0.13* |
| GPx | 0.501 ± 0.007 | 0.405 ± 0.031* | 0.417 ± 0.014* | 0.429 ± 0.008 |
| GR | 8.10 ± 0.33 | 7.68 ± 0.16 | 7.20 ± 0.25* | 8.49 ± 0.06 |
| GST | 4.70 ± 0.06 | 4.15 ± 0.11* | 4.63 ± 0.10 | 4.35 ± 0.05* |
Abbreviations and units: Reactive Oxygen Species as ROS—FIU; Reduced glutathione as GSH—mg/gm, Oxidized glutathione as GSSG—mg/gm, Thiobarbituric acid reactive substances as TBARS——μg/gm, Superoxide dismutase as SOD—units/min/mg protein; Catalase—μmoles H2O2 produced/min./mg protein; Glutathione Peroxidase as GPx—nmole conjugate produced/min/mg protein; Glutathione reductase as GR—units/l and Glutathione S-transferase as GST—μmole/min/mg protein.
Values are mean ± SE; n = 5.
*
p < 0.05 Compared to normal control group.
**
p < 0.05 Compared to normal arsenic group.