Fig. 1.

(A) Schematic of experimental design. C57bl/6n dams, n = 6–7 per treatment, were given drinking water containing BPA, 25 ng/ml, or an equal volume of ethanol (vehicle, VEH) beginning on gestation day 11.5 and continuing until weaning on postnatal day 21. The drinking water of progeny contained 2.5 ng/ml BPA beginning at weaning until euthanasia at 4 months of age. A separate group of dams were orally treated with DES (1 μg/kg/day) using a micropipette on gestation days 11.5–14.5. VEH, BPA and DES progeny were randomly selected and fed either a control (CD) or high fat diet (HFD) from weaning until euthanasia. (B–D) Physiological parameters at weaning (B) or with time on diets in male (C) and female (D) mice. BL is body length in cm; BW is body weight in grams; BMI is body mass index using the formula BMI = [BW/(BL)²] x 100 where BW is in g and BL in cm. Body surface area (BSA) was calculated using the formula$\text{BSA}=\left[\text{K}\times \sqrt[3]{{\text{W}}^2}\right]$). Data are expressed as mean ± SEM. Significance indicated by p < 0.05 when compared with * VEH; ^† CD.