Skip to main content
. 2014 Oct 22;1:843–857. doi: 10.1016/j.toxrep.2014.10.011

Fig. 7.

Fig. 7

Effects in MES 13 cells of 24 h pre-treatment of with 0.01% LipOmega-3 “9/1” on Gentamicin/Ketorolac induced cytotoxicity: Changes in apoptosis; (A) mRNA expression of apoptosis-relevant parameters BCL-2, BAX and active Caspase-3; p, significance (by t-test) nephrotoxin (Ketorolac or Gentamicin) plus 0.01% LipOmega “9/1” pre-treatment vs. nephrotoxin alone. Values are given as mean of 3–4 independent experiments. NS, not significant. (B) and (C), morphologically measured apoptosis rate. Values expressed (in % of apoptosis) are given as mean + SEM. #p ≤ 0.05, ###p ≤ 0.001 (by t-test) significance vs. control; n = 3 independent experiments. (D) and (E) Western blot of active caspase-3 protein expression, quantified by densitometric analysis using ImageJ software; values expressed in % of control (100%) are given as mean + SEM. #p ≤ 0.05, (by t-test) significance vs. control (=100%). *p ≤ 0.05, (by t-test) significance vs. nephrotoxin; (F) representative Western blot of active caspase-3. n = 4 independent measurements.