Fig. 4.

Effect of pesticides on cell layers integrity. Caco-2 cells were allowed to grow on permeable membrane filter supports in presence of pesticides at concentrations ranging from 1 to 25 μM for 10 days. Transepithelial electrical resistance (TEER) was measured at days 0, 3, 7, and 10 following proceeding described in Section 2 and its value is expressed as percent of control. In parallel, phenol red passage through cell layer was assessed at day 10 (inset) as described by Section 2. Both TEER and PR diffusion values are expressed in means ± SEM of 3 independent experiences done in triplicate. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)