Table 1.
EC50 values (μM) of pesticides alone on Caco-2 cells upon 3, 7, and 10 days-period of exposure. Caco-2 cells were exposed to DTM, FNT, FPN, LCT, and TBZ either alone or as a mixture of the five for 3, 7 and 10 days at concentrations ranging from 0.1 to 100 μM. After exposure, cell viability was assessed by MTT assay and effect concentrations 50% (EC50) were calculated according to the dose response curves in Fig. 1 as described in Section 2.
| Time (days) | EC50 (μM)a |
||||
|---|---|---|---|---|---|
| DTM | FNT | FPN | LCT | TBZ | |
| 3 | 191.0 ± 5.5 | 31.6 ± 0.7 | 24.1 ± 0.4 | 72.9 ± 2.7 | 571.1 ± 51.0 |
| 7 | 136.4 ± 10.7 | 25.6 ± 0.8 | 21.6 ± 0.5 | 74.3 ± 4.8 | 471.3 ± 37.6 |
| 10 | 111.9 ± 3.3 | 21.3 ± 0.5 | 18.1 ± 0.5 | 59.5 ± 2.3 | 429.4 ± 24.5 |
Mean ± SEM of three independent experiments.