Table 2.
Statistical comparison between measured and predicted combined effect concentrations (EC50). Caco-2 cells were exposed to the mixture of the five for 3, 7 and 10 days at concentrations ranging from 0.1 to 100 μM. After exposure, cell viability was assessed by MTT assay and predicted effect concentrations 50% (EC50) were calculated according to Section 2. IA, independent action model; CA, concentration addition; CI, confidence interval.
| Time (days) | Measured |
IA |
CA |
|||
|---|---|---|---|---|---|---|
| Mean | 95% CI | Mean | 95% CI | Mean | 95% CI | |
| 3 | 27.5 | 26.1–29.0 | 7.7 | 7.4–8.0 | 51.3 | 51.0–51.5 |
| 7 | 22.6 | 21.5–23.8 | 7.5 | 7.2–7.8 | 43.3 | 42.8–43.8 |
| 10 | 20.1 | 19.1–21.2 | 5.1 | 4.9–5.4 | 35.1 | 34.9–35.3 |