Table 3.
Summary of the effect of pesticides alone or in combination on cell viability and transepithelial membrane resistance (TEER), apparent permeability (phenol red diffusion) and alkaline phosphatase activity (ALP) at J10, on lipid peroxidation and H2O2 production (upon 48 and 6 h, respectively) and on the ration of the percentage of apoptotic or necrotic cells in treated versus untreated cells (48 h). In control cells (DMSO treated cells) the ratio of the apototic cell percentage versus necrotic cell percentage was equal to 0.5.
| Cell viability (J10)* | TEER (J10) | Apparent permeability (J10) | ALP activity (J10)** | Lipid peroxidation** | H2O2 production* | Percent of apoptosis in treated vs control cells | Percent of necrosis in treated vs control cells | |
|---|---|---|---|---|---|---|---|---|
| Deltamethrin | ↘ | ↗ | ↘ | ↗ | ↗ | ↗ | 4.3 | 2.7 |
| 8 μM | 10 μM | 25 μM | 1 μM | 100 μM | 25 μM | |||
| Fenitrothion | ↘ | ↘ | ↗ | ↘ | ↗ | ↗ | 6.3 | 4.4 |
| 5 μM | 10 μM | 25 μM | 5 μM | 25 μM | 25 μM | |||
| Fipronil | ↘ | ↔ | ↘ | ↘ | ↗ | ↗ | 8.6 | 3.2 |
| 5 μM | 5 μM | 10 μM | 25 μM | 100 μM | ||||
| Teflubenzuron | ↘ | ↘ | ↗ | ↗ | ↗ | ↗ | 3.9 | 3.8 |
| 5 μM | 5 μM | 25 μM | 5 μM | 25 μM | 100 μM | |||
| Lambda cyalothrin | ↘ | ↗ | ↘ | ↗ | ↗ | ↗ | 3.0 | 2.7 |
| 1 μM | 25 μM | 25 μM | 1 μM | 100 μM | 100 μM | |||
| Mixture | ↘ | ↗ | ↘ | ↗ | ↗ | ↗ | 8.9 | 2.7 |
| 1 μM | 10 μM | 5 μM | 1 μM | 100 μM | 25 μM |
*
p < 0.05.
**
p < 0.01.